Transformation of short-chain chlorinated paraffins by the bacterial haloalkane dehalogenase LinB – Formation of mono- and di-hydroxylated metabolites
نویسندگان
چکیده
منابع مشابه
Expression of glycosylated haloalkane dehalogenase LinB in Pichia pastoris.
Heterologous expression of the bacterial enzyme haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 in methylotrophic yeast Pichia pastoris is reported. The haloalkane dehalogenase gene linB was subcloned into the pPICZalphaA vector and integrated into the genome of P. pastoris. The recombinant LinB secreted from the yeast was purified to homogeneity and biochemically characterized...
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Despite the fact that chlorinated paraffins have been produced in relatively large amounts, and high concentrations have been found in sewage sludge applied to soils, there is little information on their concentrations in soils and the effect on soil organisms. The aim of this study was to investigate the toxicity of chlorinated paraffins in soils. The effects of short-chain chlorinated paraffi...
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Short-chain chlorinated paraffins (SCCPs) are highly complex technical mixtures of polychlorinated n-alkanes with carbon-chain lengths from C10-C13 and chlorine content between 49 and 70%. They are produced by chlorination of n-alkanes and do not occur naturally. Because of their physical properties (viscosity, flame resistance) they are used in many different applications, such as lubricant ad...
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The hydrolytic dehalogenation of rac-1,3-dibromobutane catalyzed by the haloalkane dehalogenase LinB from Sphingobium japonicum UT26 proceeds in a sequential fashion: initial formation of intermediate haloalcohols followed by a second hydrolytic step to produce the final diol. Detailed investigation of the course of the reaction revealed favored nucleophilic displacement of the sec-halogen in t...
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abstract because of the many geopolitical, geo economical and geo strategically potentials and communicational capabilities of eco region, members can expand the convergence and the integration in base of this organization that have important impact on members development and expanding peace in international and regional level. based on quality analyzing of library findings and experts interv...
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ژورنال
عنوان ژورنال: Chemosphere
سال: 2021
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2020.128288